Objective To construct a virtual simulation experiment teaching system of infectious disease nursing with viral hepatitis as an example and carry out a feasibility demonstration. Methods The virtual simulation experimental teaching system of viral hepatitis was designed and developed with the guidance of nursing process. The system was applied to the experimental teaching of nursing undergraduates. The effectiveness and feasibility of the system was evaluated by the score of virtual experiment operation,the number of operations per person,the duration of experiment and the satisfaction of students. Results The virtual simulation experimental teaching system of viral hepatitis was constructed with the diagnosis,treatment and nursing of viral hepatitis patients as the background case. According to the nursing process,it was divided into four modules:disease diagnosis,admission nursing,symptomatic nursing and discharge nursing. The four modules include a total of 14 interactive tasks,of which 5 interactive tasks set multiple paths. The average score of virtual experiment operation of 560 students was 80.02 ± 12.75 points,the total number of system usages was 698 times,used 1.25 times per person,and the average experiment duration was 61.30 ± 24.54 minutes. The overall satisfaction of students with the system was 96.96%. Conclusion The experimental teaching system constructed in this study has good effectiveness and feasibility,and can provide reference for the virtual simulation experiment teaching in infectious disease nursing for nursing undergraduates.