Objective To investigate the current status of on-the-job training load of nurses in Xiangyang and to analyze its influencing factors. Methods A questionnaire survey was carried out in 540 clinical nurses in three hospitals in Xiangyang by using the Clinical Nurses Training Load Scale and the Nursing Staff Autonomous Learning Ability Evaluation Scale. Results The score of on-the-job training load was（3.38±0.89）. Multivariate linear stratified regression analysis showed that age,education,number of children,degree of enjoying the work,hospital level,department,autonomous learning ability entered the regression equation（P<0.05）,which could explain the 42.2% total variance. Conclusion The training load of clinical nurses is at the middle level. Age,education,number of children,degree of enjoying the work,hospital level,department,autonomous learning ability are the important factors that affect the training load of clinical nurses.