Objective To explore the effects of jolly factor together with interest motivation on depression of post-stroke patients. Methods A total of 63 eligible post-stroke patients with depression were recruited from December 2015 to July 2016 in rehabilitation department of the Rehabilitation Hospital which is affiliated to the Fujian University of Traditional Chinese Medicine. Patients were recruited according to the inclusion and exclusion criteria. Thirty-one patients in Ward Ⅰ were in the experiental group,while 32 patients in Ward Ⅱ were in the control group. Both groups received routine therapy and care. Patients in the experimental group received the intervention of jolly factor together with interest motivation. Contents of the intervention included chatting with the patients,participating some activities such as handwriting,singing,storytelling,and throwing and catching balls. The Hamilton Depression Scale（HAMD） and Modified Barthel Index（MBI） were used to evaluate patients’ depression level before the intervention and 4 weeks after the intervention. Results The depression level and daily activity level of patients in the experimental group were significantly higher than those of patients in the control group（P<0.05）. Conclusion Jolly factors together with interest activation can alleviate depression level of post-stroke patients and improve their activities of daily living.