Objective To evaluate the effects of infant incubator cleaning and disinfection micro-video on training of neonatal nurses. Methods A research team was formed to develop a micro video regarding infant incubator clearning and disinfedion. A total of 126 nurses from the neonatal disease center of a tertiary children’s hospital were selected. WeChat groups were established to carry out micro-video training. At the end of the training,nurses were assessed on the knowledge and practical operation of cleaning and disinfection of infant incubator. Nurse’s examination performance before and after the training and the cleaning quality of the infant incubator were compared. Results The nurses’ learning attitude was very active and they were highly involved in the micro-video training. After the micro-video training,the performances both in examination and practical operations of nurses in were better than those before the training（P<0.05）. The cleanliness and hygiene quality of infant incubator were better than those before the training（P<0.05）. Conclusion Micro-video training can improve the knowledge level and operational ability of nurses,stimulate learning interest,and improve the effects of nursing training.