Objective To evaluate the effectiveness of the narrative pedagogy applied in the course of Obstetrics and Gynecology on caring ability of nursing undergraduates. Methods A total of 252 nursing undergraduates was recruited in the study. Narrative pedagogy was used in practice sessions of Obstetrics and Gynecology course. Caring Ability Inventory（CAI）and self-designed questionnaires were used to evaluate caring ability and feedback of students. Results After the implementation of the narrative course,score of courage subscale was higher than that before the course（P<0.05）. However,the scores of the CAI and other subscales were not statistically different before and after the implementation of the course（P>0.05）. 80.3% students believed that narrative pedagogy was helpful to cultivate their humanistic care ability. Conclusion Narrative pedagogy has positive effects on caring ability of nursing students and can be promoted in nursing education.