Objective To construct a knowledge graph for diabetic foot（DF） and assess its usability and acceptability. Methods Evidence was extracted from DF-related guidelines retrieved from guideline networks，professional association websites，and domestic/international databases to establish the initial content framework and foundational knowledge of the DF knowledge graph. Following two rounds of expert panel discussions，the final version was developed；and a web-based DF knowledge graph was developed. From November 2023 to December 2023，15 clinical nurses specializing in diabetes/DF care at a tertiary grade A hospital in Lanzhou City and 15 DF patients were invited to trial the DF knowledge graph for two weeks. Evaluations were conducted using the System Usability Scale（SUS） and the DF Knowledge Graph Experience Questionnaire，with participants also providing feedback on limitations and suggestions. Results The DF knowledge graph comprised 539 nodes，49 relationship types，and 523 inter-node links，with functionalities including knowledge query，search，and interactive Q&A. The usability scores of the DF knowledge graph were（86.00±8.33） points for nurses and（80.83±7.42） points for DF patients. In terms of user experience，nurses and DF patients scored[9（8，10）] points for usage intention，while their recommendation intention scores were 4（3，5） points and 4（4，5） points respectively. A total of six nurses and two patients provided feedback on limitations and suggestions. Conclusion The DF knowledge graph demonstrated good usability and high acceptability，serving as an effective auxiliary tool for nurses in health educationand a reliable health information platform for DF patients.