Objective To evaluate the teaching practice of a Comprehensive Humanistic Nursing Competency Training course based on authentic learning. Methods A teaching design was developed for the Comprehensive Humanistic Nursing Competency Training course based on authentic learning，including clarifying teaching objectives，determining teaching themes，designing teaching implementation，constructing teaching resources，and establishing teaching evaluation. The course was implemented from April to June 2023 among 397 undergraduate nursing students（class of 2020）. Students' humanistic care ability was assessed using the Caring Ability Inventory（CAI） before and after the course. Post-course evaluations included students' reflection diaries to assess teaching effects，teaching satisfaction surveys，and students' feedback. Results After the course，the total score of CAI and scores in the understanding and patience dimensions significantly increased compared to pre-course levels（P<0.001）. Taking the “empathy” theme as an example，analysis of reflection diaries revealed four key themes namely understanding the connotation of empathy，clarifying the role of empathy，strengthening the belief in practicing empathy，and summarizing the implementation methods of empathy. The satisfaction rates reached 99.2%（377/380）for teaching quality and 98.7%（375/380） for instructional design. Students suggested ways for further improvements including enhancing feedback on reflection diaries，increasing challenge levels in role-playing and real-case scenarios，and diversifying assessment methods. Conclusion The authentic learning-based Comprehensive Humanistic Nursing Competency Training course can effectively enhance nursing undergraduates' humanistic care abilities with high student satisfaction. Further optimizations should incorporate students'suggestions for improvement.