Establishing first-class undergraduate curriculum is a pivotal measure to enhance the quality of nursing higher education and cultivate highly qualified nursing professionals. School of Nursing of Nanjing Medical University aligns with the comprehensive requirements outlined by the Ministry of Education for constructing first-class undergraduate curriculum. This paper reported the development of online first-class curriculum，offline first-class curriculum，blended online and offline first-class curriculum，virtual simulation experiments as first-class curriculum，and first-class curriculum related to social practices. Covering a multitude of aspects，including transforming educational philosophies，innovating diverse teaching methods，optimizing educational content，enriching teaching resources，and reforming assessment methods，this article articulated the measures taken in the construction of professional curriculum. The aim of this paper is to offer insights and references for the construction of nursing curriculum at other domestic universities.