Objective To design a mixed ideological and political teaching program for undergraduate Nursing Humanities courses centered on the cultivation of professional emotions and improvement of professional qualities，and to explore its application effects. Methods A mixed ideological and political teaching program for undergraduate Nursing Humanities courses was designed，with the main steps including clarifying the ideological and political dimensions of the course and designing its ideological and political objectives；selecting ideological and political teaching topics and exploring the ideological and political elements of the course；and creating diversified micro-case resources for ideological and political education. A total of 112 nursing undergraduate students from the 2021 cohort of a medical university were selected as the research subjects. The mixed ideological and political teaching model of “triggering perception-learning empowerment-action transformation” was implemented in the Nursing Humanities course. The teaching effect was evaluated through course performance，teaching satisfaction and teaching effect survey results，as well as the semantic network analysis and sentiment analysis results of students’ replies in the ideological and political reflection topic discussion area. Results The course performance of the 112 students was（85.55±3.41） points；108（96.4%） students expressed satisfaction or great satisfaction with the teaching，and 106（94.6%），101（90.2%），and 102（91.1%） students believed that the course helped improve their nursing humanistic care awareness，empathy，and communication skills，respectively. Meanwhile，103（92.0%），99（88.4%），and 96（85.7%） students believed that the course helped enhance their professional recognition，understanding of professional value，and professional self-confidence，respectively. The results of semantic network analysis and sentiment analysis on the content of 2 167 replies indicated that two major semantic networks had been formed with “nurse” and “nursing” as the core words. Discussions on “nurse” mainly focused on working ability and working environment，while discussions on “nursing” mainly concentrated on internal professional perception and external professional evaluation. Among the replies，the number of texts expressing positive emotions accounted for 916（78.90%）. Conclusion The mixed ideological and political teaching program for Nursing Humanities courses not only helps enhance students’ learning satisfaction and sense of achievement，but also stimulates their professional emotions and improves their professional qualities.