Objective Based on the construction an OSCE case base for the teaching of surgical nursing practice based on the Association for Medical Education in Europe Guide No. 81（AMEE Guide No. 81），this study aims to explore its usage for standardizing the construction of an OSCE case base for teaching surgical practice in the future. Methods Based on the AMEE Guide No. 81 guidance，the case library construction was carried out from December 2021 to January 2023，including the formation of an expert review panel，the drawing of an authoring framework，the identification of authoring topics，the selection of an authoring team，and the development of a case library template. Then，we invited 19 experts for two rounds of correspondence using the Delphi method to revise the OSCE case base based on the experts’ opinions. Finally，we analyzed the results of the case quality scores from the 2nd round of consultation. Results The study has developed a surgical practice teaching OSCE case base consisting of 16 categories of surgical nursing practice teaching OSCE specialty cases. Each category of case was based on a developed authoring template that included six specific sections：information about the examination station，candidate guidelines，examiner guidelines，standardized patient instructions，assessment supplies and equipment，and other information about the case. Quality scores for each case were ranged from 87.63 to 91.47. Conclusion Based on AMEE Guide No. 81，a high quality OSCE case bank for teaching surgical nursing practice is suitable for the teaching in surgical nursing clinical practice.