Objective To explore the effect of general education course of Multiculture and Nursing，so as to provide reference for the implementation general nursing education in comprehensive universities. Methods Multicultural and Nursing general courses was desigened from the international and cross-cultural perspective，with integrated teaching content of “nursing+multicultural”. Course construction was carried out through literature review，expert consultation and demonstration. Information-based auxiliary teaching was implemented with the network course platform as the main body and the network interaction and nursing skill practice virtual simulation platform as the supplement，endowing the course with diversity，practicality and high-level. In the second semester of the 2016 academic year，the course began to be avaialbe for all the undergraduate students with different majors. A total 589 students were recruited to conduct a questionnaire survey and to analyze the students’ course selection，learning evaluation and teaching effects. Results Non-medical majored students accounted for 88.29% of the total participants. In addition，53.99% of the students most interested in medical knowledge and skills. Students wrote 589 research essays，completed 60 research reports，and scored 83.26 points on average. The total score of the students’ humanistic literacy questionnaire and the scores of humanistic knowledge，thought and method were significantly improved（P<0.05）. Besides，81.83% of the students approved of the information-based auxiliary teaching method，50.76% of the students agreed the interaction between teachers and students as well as among classmates. The overall good rate of students’ evaluation of teaching quality reached 84.55%. Conclusion The teaching practice of general course Multiculture and Nursing in comprehensive university achieves satisfactory results，and the course practice can effectively promot the improvement of students’ humanistic and medical literacy.