Objective To explore the effect of application of a new loose-leaf teaching materials during the nursing safety training course of newly-graduated nurses. Methods Based on loose-leaf teaching materials and nursing safety training course，standard operation videos and nursing adverse event case videos were developed. According to time of employment，nurses admitted in September 2019 were assigned to the control group（n=43） and those admitted in September 2020 were recruited in the experimental group（n=54）. In the control group，trainings were carried out with conventional teaching methods（self-made PPT courseware and other forms）. In the experimental group，the new loose-leaf teaching materials were applied. Comparisons were made between the two groups of nurses after the course，including their levels of satisfaction about the training methods，their grades in the theory test and operation test three months later，and the occurrence of adverse nursing events caused by violation of the core nursing system during the two-year standardized training period of new nurses. Results Compared to the control group，nurses in the experimental group had higher levels of satisfaction about the training methods，higher grades in the theory test and operation test three months later（P<0.05）. There were two adverse nursing events caused by violation of the core nursing system in the experimental group，while 14 events in the control group. Conclusion Compiling the new loose-leaf teaching materials to meet the demands of clinical nursing safety training is beneficial for the newly-graduated nurses，which can effectively improve their nursing safety awareness and standardization of operations.