Objective To explore the development method and application effect of nurse hierarchical training and management system. Methods The hierarchical training and management system for nurses was developed according to the ADDIE teaching design model and based on the key links of teaching，namely “teaching，examination，evaluation and management”. From March 2021，more than 3 600 nurses were trained with the hierarchical training and management system. By August 2022，3 016 theoretical training courses had been published，3 202 operational training courses had been organized，and 4 530 nursing ward rounds，1 976 operational examinations and 389 theoretical examinations had been completed. In August 2022，a self-made system satisfaction questionnaire was used to investigate the satisfaction of clinical nurses and teaching management personnel. Results A total of 725 nurses were investigated. The average score of satisfaction items was 4.52±0.81，and the average scores of design and operation，content setting，use effect，and use intention dimensions were 4.47±0.78，4.59±0.86，4.57±0.95，and 4.48±1.08 respectively. Conclusion The overall satisfaction of nursing staffs with the training and management system was high，which improves the convenience and flexibility of training，strengthens the quality management of training，and can provide reference for the information construction of stratified training and management of nurses.