Objective To explore the feasibility of developing high simulation training cases of physician-nurse cooperation in Obstetrics and Gynecology. Methods The cases were developed through the process of setting up the case preparation team，collecting cases，revising and reviewing cases. After that，five clinical medical students and 13 nursing students were recruited as particiapnts. Two cases of normal delivery and vaginal delivery postpartum hemorrhage were examined in the pilot study. Each practical training course lasted three class hours，with a total of six class hours. Evaluation in the feasibility of the case by understanding students’ views on the case design and teachers’ reflection on the training process. Results The students thought that the training could strengthen and deepen their understanding of roles between majors and enhance their interest in learning. The teachers believed that the design elements in the case could be realized. Conclusion It is feasible to develop and apply the high simulation training case of nursing cooperation in Obstetrics and Gynecology and can provide a reference for cultivating students’ cross-professional cooperation ability in the future.