Objective To explore the effects of the blended teaching in Thinking and Communication course on the cultural competence of nursing undergraduates. Methods From October 2021 to January 2022，two classes were assigned to the experimental group（n=169） and the control group（n=168） by tossing a coin. The blended teaching that combined online and traditional classrooms teaching was applied in the experimental group，while the routine teaching method was applied in the control group. The Chinese version Inventory for Assessing the Process of Cultural Competence among Health care Professionals（IAPCC-CV） and theoretical examination were used to evaluate the effects. Results Before the course，the cultural competence scores of the experimental group and the control group were（65.89±5.23） and（65.65±5.30） respectively，and the difference was not statistically significant（t=0.427，P=0.670）. After the course，the cultural ability scores of the experimental group and the control group were（71.91±8.49） and（68.73±7.71） respectively（t=3.597，P<0.001）. The theoretical scores of the experimental group（91.64±5.21）were higher than those of the control group（88.44±7.85）（t=4.407，P<0.001）. Conclusion The blended teaching in Thinking and Communication course can improve the teaching effects and strengthen the students’ mastery of knowledge.