Objective To explore the construction method and teaching effect of Hospice Nursing course for nursing undergraduates. Methods Based on surveys and expert consensus，the specialized and optional course of Hospice Nursing was set up and improved. The teaching objective of the course was to guide students to establish correct attitude to life and death，master the knowledge and skills of hospice nursing，and improve their attitudes and abilities in hospice care. The teaching contents included death education，quality of life evaluation，common symptom management，psychological care of dying patients and their families，etc. Diverse teaching methods were adopted，such as case teaching，scenario simulation teaching，debate and etc. Formative evaluation was used for assessment. A total of 35 nursing undergraduates of 2018 Class were selected as the participants，and the teaching effect was evaluated by the course scores，the interview of students’ teaching satisfaction，and the surveys of students’ attitude toward death anxiety and hospice care. Results The course scores of 35 students were 87.43±0.84. In interview，18 students were satisfied with the content，method，assessment and experience of teaching，and put forward suggestions to increase teaching hours and carry out clinical practice. After taking the course，the total score of death anxiety among students was lower（Z=3.248，P<0.001），and the total score of hospice attitude was higher（t=5.284，P<0.001）than before. Conclusion These students passed the Hospice Nursing course examination smoothly and were satisfied with the teaching effect. Besides，their death anxiety got reduced with improved attitude toward hospice care.