This paper introduced the development and application of the diversified evaluation program in the Advanced Nursing Practice in School of Nursing,School of Medicine,Soochow University. According to the training orientation of master of nursing specialist,the objectives of Advanced Nursing Practice and the characteristics of teaching content,a diversified evaluation program with 4 aspects including practical assessment,humanistic quality activities,comprehensive classroom performance and reflective diary was constructed through literature research and expert group meetings. The program was used to evaluate knowledge,skills,attitudes,emotions and other aspects of MSN graduate students. The scores of practice assessment,humanistic quality activities,comprehensive classroom performance and reflection diary of the 48 students of grade 2019 were（87.68±3.45）,（9.52±0.65）,（8.90±0.97） and（90.17±2.05）,respectively. The feedback to the diversified evaluation program was good. This diversified evaluation program can stimulate students’ autonomy and effective reflection in learning,enhance students’ classroom participation,and cultivate their humanistic qualities. It can provide references for the design and improvement of evaluation programs for other colleges and universities.