Objective To compare and analyze the characteristics of interactive behaviors in the flipped classroom and the traditional classroom. Methods Nursing students of Grade 2017 in Nanchang University were assigned to the control group and traditional classroom teaching was used in this group. Students in Grade 2018 were assigned to the experimental group and flipped classroom teaching was used in this group. The chapter of “care of patients with heart failure” in the Medical Nursing was chosen and the whole teaching process was recorded by video. The behaviors of teachers and students in the two groups were analyzed by using NVivo 11.0 software and behavior coding system. Results The proportion of teacher’s speech,student’s speech,classroom silence and technology of the total behaviors in the experimental group were 35.09%,48.75%,4.89% and 11.27%,respectively. The proportion of teacher’s speech,student’s speech,classroom silence and technology of the total behavior in the control group were 84.65%,8.19%,1.13% and 6.04%,respectively. There were significant differences between the two groups（P<0.05）. There were statistically significant differences in the specific behavior ratios of teacher’s speech,students’ speech and technical behavior（P<0.05）,while there were no statistically significant differences in the specific behavior ratios of classroom silence（P=0.062）. Conclusion There are differences in the specific behavior rates of teacher’s speech,students’ speech and technical behaviors between the traditional classroom and the flipped classroom teaching,while the proportion of classroom silence in the flipped classroom and the traditional classroom teaching is not different.