Objective To evaluate the effects of interactive group learning in stratified nurse training. Methods Thirty-eight nurses in the digestive department were trained by stratified nurse training in 2013 and trained by interactive group learning based on stratified nurse training in 2014. Then the performance evaluations and self-eval-uation of all these nurses were conducted. Results The application of interactive group learning improved the score of young nurses and senior nurses，and strengthened their personal traits，clinical nursing skills，communication skill， ability of critical clinical thinking and professional skills. Conclusion The interactive group learning helps to improve the effect of stratified nurse training and can strengthen the professional competence.