Objective To explore the effects of using a reflection prompting card in ICU new nurses training. Methods From September 2019 to January 2021,new nurses who received standardized training in ICU of a tertiary hospital in Shanghai were selected in the study. Reflection prompting cards were translated and revised,and the nurses were instructed to write work diary according to the reflection prompting card during the training process. Nurses reflected their practice on the clinical learning thinking,clinical environment and clinical behavior and recorded the new clinical knowledge every week. Results After the application of reflection prompting cards,the contents of work diaries were richer. Teachers evaluated the training effects and adjusted the teaching plans according to nurses’ diaries. The training experience of nurses were also improved. Conclusion By using reflection prompting cards,nurses can improve their independent learning and growth. The study can provide a basis for teachers to carry out individualized training.