Objective To develop the Chinese version of Nursing Student’s Knowledge about Breastfeeding Questionnaire and examine its reliability and validity. Methods According to the Brislin’s translation model,we followed the forward-translation and back-translation procedure to translate the questionnaire into Chinese,modified it based on Chinese culture. The Chinese version was revised through experts’ review and a pilot study. The convenience sampling method was used to investigate 134 nursing students. The reliability and validity of the questionnaire were tested by exploratory factor analysis,content validity,Cronbach’s α,and retest reliability. Results The final Chinese version of the Nursing Student’s Knowledge about Breastfeeding Questionnaire is a single-dimensional questionnaire with 14 items. The content validity of the total questionnaire was 0.963,and each item was 0.710~1. Cronbach’s α coefficient of the total questionnaire was 0.763,and each item was 0.613~0.752. The split-half reliability was 0.780,and the retest reliability was 0.813. Exploratory factor analysis extracted 4 common factors,and the cumulative variance contribution rate was 55.266%. Conclusion The Chinese version of Nursing Student’s Knowledge about Breastfeeding Questionnaire was demonstrated to be reliable and valid among nursing students.