Objective To explore the methods and effects of training and using clinical nursing teachers in a general hospital. Methods The special position for clinical nursing teachers was set up. The position requirements,responsibilities,role function,and remuneration were all clarified. The training program was developed based on the education procedure and provided to the 43 nurses. After qualification,these nurses were investigated by the self-developed questionnaires of workload,working ability and teaching quality. Results The annual workload score of 43 nurses was 88.73±1.82. After the training,the score of teaching ability was 85.63±2.26,which was higher than that before the training（74.51±2.18）（t=3.37,P=0.030）. After the training and use of clinical nursing teachers,the score of teaching quality of 43 nursing units was 90.21±1.89,which was higher than that before the training（84.82±2.57）（t=2.66,P=0.040）. Conclusion The standardized training,use and management of clinical nursing teachers are helpful to improve the teaching level and quality of the whole hospital and promote the development of clinical nursing teaching.