Objective To explore the application effect of on-site + live broadcast teaching pattern in Anatomy experiment teaching of nursing specialty in higher vocational colleges. Methods Four first year classes were randomly selected,which were from a university’s higher vocational nursing classes. Two of the classes with totally 85 students were divided into 4 batches and they were in the experimental group in which the on-site + live broadcast teaching pattern was adopted and the live video duration was（29.9±1.3） minutes;the other two classes with a total of 86 students were in the control group in which the teaching in group by demonstration teaching pattern was used. The teaching effect was evaluated by comparing the two groups’ classroom experimental test scores,experimental test scores in two weeks after the class and the comments of the experimental group about the teaching pattern. Results Both the classroom experimental test scores and the experimental test scores two weeks after the class of the experimental group were higher than those of the control group（t=4.262,9.177,P<0.001）. The experimental group held these opinions：the teaching pattern was novel（84/85）;the teaching effect was good（81/85）;it was convenient to use the teaching pattern（84/85）;they were able to see the small structure（74/85）;they would review the live video after the class（81/85）;they were willing to accept the on-site and live broadcast teaching pattern（82/85）. Conclusion In the course of anatomy experiment teaching of nursing specialty in higher vocational colleges,the on-site + live broadcast teaching pattern can display the small structure and it is convenient for the students to observe the experiment in class and to review it at any time after class,which can effectively improve the experimental teaching effect of orthopedics.