Objective To explore the methods and effects of training for midwives with different levels. Methods A total of 68 midwives in the obstetric departments of 2 tertiary hospitals were selected. Before the intervention,the Midwifery Competency Assessment Tool was used to assess their ability and they were divided into the novice level,competency level,proficiency level,and expert level according to their scores. Based on the constructed midwife-level training program,they were trained for 2 years. Level adjustment was performed based on the assessment results. The theoretical and practical assessment results were compared before and after intervention. The level changes were also evaluated. Results After the training,the scores of the theoretical examination and the scores of various practical skills of the midwives at all levels were improved compared with those before the training（all P<0.05）.The number of midwives of novice level was reduced from 32 to 13,the number of competency level was increased from 22 to 30,the number of proficiency level was increased from 12 to 21,and the number of expert level was increased from 2 to 4. Conclusion Level training of midwives can improve the theoretical and operational capabilities of clinical midwives at all levels,and achieve dynamic management.