Objective To establish the evaluation index system of the network teaching platform based on nursing cases database. Methods Two theories of usability evaluation and web-based learning environment usefulness evaluation framework were used as the theoretical framework. The evaluation index system of nursing cases database network teaching platform was initially formed by literature analysis. Through two rounds of expert consultation and analytic hierarchy process,the index system and weight were finally determined. Results The index system included 3 categories(platform usability,teaching utility and additional utility),11 subcategories and 39 items. The recovery rate of the two rounds of questionnaires was 100% and the authoritative coefficient was 0.85. The first round Kendall's W coordination coefficient was between 0.121 and 0.326,and the second round was between 0.210 and 0.326(P<0.05). Conclusion The index system is reasonable in design and the index weight are acceptable. The index system can be used as an evaluation tool for network teaching platform based on nursing cases database.