Objective To explore the teaching effects of blended gold course in the course of Fundamentals of Nursing. Methods The nursing undergraduates in Grade 2017(n=211) were recruited in the experimental group,and the blended teaching of gold course was adopted in this group. Teaching reforms were carried out from pre-teaching design,creation of teaching situations,mixed teaching activities,and course assessment and evaluation based on the“Gold Course” standard. Blending learning includes pre-class self-study,classroom interaction and after-class consolidation. The students in Grade 2016(n=238) were assigned to the control group and traditional teaching was used in this group. After the course,students' online learning completion of the experimental group was evaluated. Course scores,students' independent learning ability and teaching satisfaction were compared between the two groups. Results The times of platform login in the experimental group were over 100 000,and the completion rate of students' learning task was over 95%. The final exam score,total scores and scores for each dimension of self- learning ability,degree of teaching satisfaction of students in the experimental group were higher than those of students in the control group(P<0.01). 89.10% of the students were satisfied with the teaching. Conclusion The teaching mode of blended gold course has achieved good effects in the theoretical teaching of Fundamentals of Nursing. Teachers should perform the reform of hybrid teaching model according to gold course standard,and carry on the all-round curriculum construction during teaching process,so as to create a gold course with “high order,innovations and challenge”.