Objective To understand the personal learning needs of online blended teaching of nursing undergraduates. Methods An expert consultation questionnaire was developed based on the needs analysis theory. Five experts were consulted for two rounds and the final questionnaire included 4 first-level dimensions,12 second-level dimensions,and 26 items. A total of 151 year-two and year-three nursing undergraduates were investigated with this self-developed questionnaire. Results The top three contents that students needed for online learning were course preparation（6.35）,knowledge explanation（6.19）,and interest stimulation（6.03）. 65.6% of the students preferred “inquiry learning” format for the online learning. 43.1% of the students believed that “the best length of online learning for a single session was 1~<2 h/d”. 43.2% of the students believed that “the length of a single learning video was 5~<10 min”. Students thought that the top three important evaluation methods were online homework（6.21）,online examination（6.18）,and online learning length（5.87）. Conclusion Teachers should pay attention to online learning “course preparation”,strengthen inquiry-based learning,reasonably arrange online teaching length to avoid study burden,optimize the types and length of online teaching resources,and carry out diversified online teaching evaluations.