Objective To develop a questionnaire of Knowledge-Attitude-Practice of Ward Hospital Infection Management and to test its validity and reliability. Methods Based on Regulation for Healthcare Associated Infection Control in Ward in Healthcare Setting,a variety of guidelines and the knowledge-attitude-practice model,items pool was formed. The first version of the questionnaire was developed by Delphi and pre-investigation.A questionnaire survey was conducted among 2 300 medical and nursing staff to test its reliability and validity. Results The formal questionnaire consisted of 3 dimensions and 52 items.The Cronbach’s α coefficient was 0.978,and the test-retest reliability was 0.928 after 2 weeks. 6 common factors were obtained, and the cumulative variance contribution rate was 76.23%. Conclusion The questionnaire has good reliability and validity,and can be used as a tool to evaluate the knowledge,belief and behavior level of hospital infection management of medical staff in general wards.