This paper mainly introduced the main process of the case development of physician-nurse cooperation simulation-based learning for master of nursing specialist students in our school,including the establishment of a case preparation team,collection of clinical cases,set teaching goals,case editing,and formation of scripts and application. And the cases are set up for medical students and nursing students,as well as medical and nursing cooperation. It is pointed out that when preparing cases,attention should be paid to meeting the needs of different professions,and reflecting the cooperation of medical and nursing care. Besides,trying to choose real cases as the source of cases is also suggested. The degree of difficulty of cases should be compatible with the students’ theoretical and practical level.