Objective To compare the effects of different training methods on personal protective skills of healthcare professionals dealing with epidemic infectious diseases. Methods A total of 138 physicians and nurses were recruited and divided into the A,B and C group randomly. The trainings were conducted according to the guidelines of Chinese Center for Disease Control（CDC） and World Health Organization （WHO）. Healthcare professionals in group A received the training of “picture illustration and skill demonstration”. Healthcare professionals in group B received another training strengthening these points which were easily missed besides contents in group A. Group C received “number highlighted” besides the contents of group B. The contamination spots and the time duration of putting off the personal protective equipment（PPE） were compared among the three groups. Results There were no significant differences in the number of contamination spots on the body surface among the three groups（P>0.05） after removal of PPE according to the guidelines of Chinese CDC. However,the number of contamination spots in group B and C was significantly less than that in group A after the removal of PPE according to WHO guideline （P<0.05）. Time duration of removing PPE in group C was significantly shorter than that in group A and B （P<0.05）. Conclusion Each training method has its own advantages and limitations. Hospitals can improve the knowledge,attitude,and behaviors of their healthcare professionals with consideration of the training resources,human resources,material resources and other conditions.