Objective To explore the effects of PICC training on knowledge and compatible skills of nurses within the medical partnerships. Methods The training was developed into 4 parts including theoretical knowledge,practical ability,skill performance,and real work performance according to results of previous analysis. Thirty-six nurses were recruited from the medical partnerships. After the training,their knowledge,skills,and satisfaction with the training were compared with another 36 nurses of the tertiary hospitals. Results The score of basic knowledge and skill after the training were significantly higher than those before the training（P<0.001）. The training satisfaction rate reached 95.7%. There were no significant differences regarding the knowledge,skills,and satisfaction with the training between nurses from the medical partnerships and their counterparts（P>0.05）. Conclusion The PICC training of nurses can effectively improve the score of basic knowledge,skill,the training satisfaction of nurses from the medical partnerships. The training can help these nurses achieve the same level of nurses from tertiary hospitals.