Objective To explore the effects of COVID-19 Micro-lecture training for ICU healthcare professionals. Methods The teaching group was established by the local intensive care alliance. The specific contents regarding prevention and control of COVID-19 were screened and selected. All healthcare professionals in ICU of the whole city were required to learn these contents online and attend the examination. Feedback of the training was collected through questionnaire. Results Totally 508 ICU medical staff gave their feedback and 466（91.7%） healthcare professionals completed the whole sessions of the training. The average satisfaction rate regarding the training duration,contents,significance,skills,gains,and feelings was 95.7%. Conclusion The online Micro-lecture which is conducted by the intensive care quality control center has good teaching effects for the main staff who are battle with the virus.