Objective To establish a training program for nursing teachers in the operating room.Methods The on-job training program was developed based on the literature review and consultation with 12 experts. Two-round consultation with 15 experts were made by Delphi method and the training program were finalized. Meanwhile,contents,study hours,and teaching methods were all established.Results Four training items were established including professional qualities,teaching abilities,knowledge,and research ability. Twenty detailed contents were involved under these 4 items. A total of 40 hours were needed. Teaching methods contained lecture,bedside teaching,re-demonstration,scenario,and case discussion.Conclusion The training program for nursing teachers in the operating room is scientific. It can provide reference for standardizing the training and improving the competence of the teaching staff.