目的 构建护士配合机器人辅助手术（robot-assisted surgery，RAS）的虚拟仿真培训系统，并探讨其应用成效。 方法 通过问卷调查、半结构化访谈及专家会议法，构建护士RAS配合培训方案，基于该方案开发相应的虚拟仿真培训系统。选取于云南省某三级甲等医院参加2024年云南省手术室专科护士培训的54名护士作为研究对象，通过抽签法，将其分为对照组（n=27）和试验组（n=27）。依照护士RAS配合培训方案，试验组接受融入虚拟仿真项目的培训，对照组接受常规培训。比较两组理论知识考核成绩、模拟操作技能考核成绩与操作时长、案例分析考核成绩、手术配合错误发生次数，以及手术医生对其的满意度。 结果 培训后试验组理论知识考核成绩、模拟操作技能考核成绩与操作时长、案例分析考核成绩、9种手术配合错误发生次数及手术医生对其的满意度优于对照组（P<0.05）。 结论 护士配合RAS的虚拟仿真培训系统具有实用性，有利于提高护士RAS配合知识与技能水平及手术医生的满意度，具有一定的借鉴价值。

Objective To construct a virtual simulation training system for robot-assisted surgery （RAS） nursing cooperation and explore its application effect. Methods A RAS nursing cooperation training program was constructed through questionnaires，semi-structured interviews and expert meetings，and a corresponding virtual simulation training platform was developed based on this program. A total of 54 nurses who participated in the 2024 Yunnan Provincial Operating Room Specialty Nurse Training in a tertiary first-class hospital in Yunnan Province were selected as the participants，and they were divided into the control group（n=27）and an experimental group（n=27） through lottery. In accordance with the RAS nursing cooperation program，the experimental group received training integrated with virtual simulation programs，while the control group received conventional training. The two groups were compared in terms of theoretical knowledge assessment scores，simulated operation skill assessment scores and operation duration，case analysis assessment scores，the number of surgical cooperation errors，and the satisfaction of surgeons with them. Results After training，the experimental group was better than the control group in theoretical knowledge assessment scores，simulated operation skill assessment scores and operation duration，case analysis assessment scores，the number of nine types of surgical cooperation errors，and the satisfaction of surgeons with them（P<0.05）. Conclusion The RAS nursing cooperation virtual simulation training system is practical，which is conducive to improving the level of nurses’ RAS nursing cooperation capabilities and the satisfaction of surgeons，and has certain reference value.