目的 基于生成式人工智能构建护理信息学智能问答系统并进行应用测试。方法 采用网络爬虫技术获取数据，构建护理信息学知识库，以增强DeepSeek-V3模型输出内容的可靠性。基于fastGPT平台实现系统功能集成，开发护理信息学智能问答系统，并开展实验测试。2025年4月—5月招募30名护理学专业学生，通过问卷调查和半结构化访谈对系统进行可用性和满意度评价。结果 实验测试结果表明，与DeepSeek-V3相比，护理信息学智能问答系统回答准确率提升15.39%，语义相似度提升3.08%。在界面清晰性、系统易用性、回答准确性和可靠性方面，学生持积极态度且意见较为一致，平均满意度得分为（4.33±0.48）分。学生访谈结果显示，该系统有助于提高知识获取效率，可以借助其更好地了解护理信息学研究动态和学术交流信息，但在知识库内容和系统功能方面仍有改进空间。结论 护理信息学智能问答系统具有良好的有效性和可用性，未来可在护理教育实践中进一步投入使用，推动护理教育的数字化和智能化转型，提升教学质量。

Objective To construct a nursing informatics intelligent question-answering system based on generative artificial intelligence and conduct preliminary study for applying and testing. Methods Data was collected using web crawling technology to build a nursing informatics knowledge base，enhancing the reliability of the content output by the DeepSeek-V3 model. The system functions were integrated using the fastGPT platform to develop the nursing informatics intelligent question-answering system，and experimental testing was conducted. From April to May 2025，30 nursing students were recruited to evaluate the system’s usability and satisfaction through questionnaires and semi-structured interviews. Results Experimental test results showed that compared to Deep-Seek-V3，the nursing informatics intelligent question-answering system improved answer accuracy by 15.39% and semantic similarity by 3.08%. Students expressed positive attitudes and consistent opinions regarding interface clarity，system usability，answer accuracy，and reliability，with an average satisfaction score of（4.33±0.48） point. Besides，interview results indicated that the system could improve knowledge acquisition efficiency and facilitates better understanding of nursing informatics research trends and academic exchange information. However，there was still room for improvement in terms of the content of the knowledge base and system functionality. Conclusion The nursing informatics intelligent question-answering system demonstrates good effectiveness and usability. In the future，it can be further implemented in nursing education to promote the digital and intelligent transformation of nursing education and enhance teaching quality.