建设护理一流本科课程是提高护理高等教育质量、培养高素质护理专业人才的重要举措。南京医科大学护理学院对标教育部关于一流本科课程建设的总体要求，对线上一流课程、线下一流课程、线上线下混合式一流课程、虚拟仿真实验一流课程及社会实践一流课程的建设进行探索与实践。该文从转变教育理念、创新多元教学方法、优化教育内容、丰富教学资源及改革成绩评定方式等方面阐述专业课程建设的举措，以期为国内其他高校护理专业课程建设提供借鉴和参考。

Establishing first-class undergraduate curriculum is a pivotal measure to enhance the quality of nursing higher education and cultivate highly qualified nursing professionals. School of Nursing of Nanjing Medical University aligns with the comprehensive requirements outlined by the Ministry of Education for constructing first-class undergraduate curriculum. This paper reported the development of online first-class curriculum，offline first-class curriculum，blended online and offline first-class curriculum，virtual simulation experiments as first-class curriculum，and first-class curriculum related to social practices. Covering a multitude of aspects，including transforming educational philosophies，innovating diverse teaching methods，optimizing educational content，enriching teaching resources，and reforming assessment methods，this article articulated the measures taken in the construction of professional curriculum. The aim of this paper is to offer insights and references for the construction of nursing curriculum at other domestic universities.