目的 设计及编制《患者安全课程思政教学指南》，并探讨其初步应用效果。 方法 以重庆医科大学患者安全课程建设为基础，以教育部《医学人文课程思政教学指南编写要求》为依据，组建编制团队，基于文献研究、专题讨论构建指南初稿，经12名专家论证后修改完善指南内容，形成《患者安全课程思政教学指南》。在2022年秋学期开课时随机选取2个小班的三年级护理本科生（n=80）应用该指南进行教学，在课程结束时采用问卷调查课程思政教学满意度；同时开展教师座谈会，讨论应用该指南进行授课的教学感受及对该指南的评价反馈。 结果 《患者安全课程思政教学指南》分为总论、各章节课程思政教学指南、附录3个部分；绝大部分学生对应用该指南教学表示满意；15名授课教师对该指南均持肯定态度，认为其具有较高的操作性、实用性和科学性，提出需加强思政元素挖掘、开展素材选取方面的培训等建议。 结论 编制的《患者安全课程思政教学指南》内容全面，科学、合理且具有可行性，能够为授课教师提供从理论原则、实际教学到评估评价的全程教学指导与参考；在教学实践中得到了验证，获得了学生和教师的一致好评。

Objective To design and compile the guideline for Ideological and Political Teaching of Patient Safety Course and explore its preliminary application effects. Methods Based on the construction of the patient safety course of Chongqing Medical University and the requirements for compilation of Ideological and Political Teaching Guideline for Medical Humanities Courses issued by Ministry of Education, a compilation team was established to construct the first draft of the guideline based on literature research and special discussions, and then revised and improved the guideline after expert argumentation(n=12), and the guideline for Ideological and Political Teaching of Patient Safety Course was formed. Two small classes of third-year nursing undergraduate students(n=80) was randomly selected at the beginning of the fall semester of 2022 to apply this guideline. At the end of the course, a questionnaire was used to investigate the degree of satisfaction with the ideological and political teaching of the course; at the same time, a teacher symposium was held to discuss the application of this guideline. Feelings about teaching ideological and political courses and feedback on the guideline. Results The Guidelines for Ideological and Political Teaching in Patient Safety Course was divided into three parts：the general introduction, the ideological and political teaching guides for each chapter, and the appendix; the vast majority of students were satisfied with the application of this guideline in teaching; 15 teachers were positive about the guideline attitude, believing that it has high operability, practicality and scientific nature, and put forward suggestions such as strengthening the exploration of ideological and political elements and carrying out training in material selection. Conclusion The Guideline for Ideological and Political Teaching of Patient Safety Course is comprehensive, scientific, reasonable and feasible. It can provide teachers with full teaching guidance and reference from theoretical principles, practical teaching to assessment and evaluation; it has been verified in teaching practice, won unanimous praise from students and teachers.